[Omeo33] Art 1136 - Homeopathy, 2010, 99 (3), 177-182

[Gino Santini]

Heparin-binding epidermal growth factor expression in KATO-III cells  
after Helicobacter pylori stimulation under the influence of strychnos  
Nux vomica and Calendula officinalis
Roland Hofbauer, Eva Pasching, Doris Moser and Michael Frass

Introduction - Previous studies have shown the stimulating effect of  
Helicobacter pylori on the gene expression of heparin-binding  
epidermal growth factor (HB-EGF) using the gastric epithelial cell  
line KATO-III. Strychnos Nux vomica (Nux vomica) and Calendula  
officinalis are used in highly diluted form in homeopathic medicine to  
treat patients suffering from gastritis and gastric ulcers.
Aim and method - To investigate the influence of Nux vomica and  
Calendula officinalis on HB-EGF-like growth factor gene expression in  
KATO-III cells under the stimulation of H. pylori strain N6 using real- 
time PCR with and without addition of Nux vomica and Calendula  
officinalis as a 10c or 12c potency.
Results - Baseline expression and stimulation were similar to previous  
experiments, addition of Nux vomica 10c and Calendula officinalis 10c  
in a 43% ethanolic solution led to a significant reduction of H.  
pylori induced increase in gene expression of HB-EGF (reduced to 53.12  
± 0.95% and 75.32 ± 1.16% vs. control; p < 0.05), respectively. Nux  
vomica 12c reduced HB-EGF gene expression even in dilutions beyond  
Avogadro’s number (55.77 ± 1.09%; p < 0.05). Nux vomica 12c in a 21.5%  
ethanol showed a smaller effect (71.80 ± 3.91%, p < 0.05). This effect  
was only be observed when the drugs were primarily prepared in  
ethanol, not in aqueous solutions. The data suggest that both drugs  
prepared in ethanolic solution are potent inhibitors of H. pylori  
induced gene expression.

Disponibile il full-text su richiesta
-------------- parte successiva --------------
Un allegato HTML è stato rimosso...
URL: http://sf8.starfarm.it/pipermail/omeopatia33/attachments/20101223/a5970b0b/attachment.html