[Omeo33] Art 0769 - eCAM, 2007, doi:10.1093/ecam/nem082

[Gino Santini]

Dynamized Preparations in Cell Culture
Ellanzhiyil Surendran Sunila, Ramadasan Kuttan, Korengath Chandran 
Preethi and Girija Kuttan

Although reports on the efficacy of homeopathic medicines in animal 
models are limited, there are even fewer reports on the in vitro 
action of these dynamized preparations. We have evaluated the 
cytotoxic activity of 30C and 200C potencies of ten dynamized 
medicines against Dalton's Lymphoma Ascites, Ehrlich's Ascites 
Carcinoma, lung fibroblast (L929) and Chinese Hamster Ovary (CHO) 
cell lines and compared activity with their mother tinctures during 
short-term and long-term cell culture. The effect of dynamized 
medicines to induce apoptosis was also evaluated and we studied how 
dynamized medicines affected genes expressed during apoptosis. Mother 
tinctures as well as some dynamized medicines showed significant 
cytotoxicity to cells during short and long-term incubation. 
Potentiated alcohol control did not produce any cytotoxicity at 
concentrations studied. The dynamized medicines were found to inhibit 
CHO cell colony formation and thymidine uptake in L929 cells and 
those of Thuja, Hydrastis and Carcinosinum were found to induce 
apoptosis in DLA cells. Moreover, dynamized Carcinosinum was found to 
induce the expression of p53 while dynamized Thuja produced 
characteristic laddering pattern in agarose gel electrophoresis of 
DNA. These results indicate that dynamized medicines possess 
cytotoxic as well as apoptosis-inducing properties.

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