[Omeo33] Art 0955 - Exp Biol Med, 2008, 233 (12), 1591-1601

[Gino Santini]

In Vitro Studies Demonstrate Anticancer Activity of an Alkaloid of 
the Plant Gelsemium sempervirens
Soumya Sundar Bhattacharyya, Sushil Kumar Mandal, Raktim Biswas, 
Saili Paul, Surajit Pathak, Naoual Boujedaini, Philippe Belon and 
Anisur Rahman Khuda-Bukhsh

The chemical structure of the main fluorescenting compound in the 
ethanolic extract (mother tincture) of the American yellow jasmine, 
Gelsemium sempervirens, was determined by employing 1H nuclear 
magnetic resonance (NMR), 13C NMR, mass spectroscopy, 
high-performance liquid chromatography (HPLC), correlation 
spectroscopy (COSY), and Fourier transform infrared (FTIR) 
spectroscopy analyses. Spectrofluorometric analysis has been made of 
the mother tincture and its agitated serial dilutions (up to 12th 
potency) prepared according to a homeopathic procedure in which 
serial, agitated dilutions were made separately in glass and 
polypropylene containers. The succussions were made by employing 
three different modes: hand jerk, sonication, and vortexing. The 
chemical formula of scopoletin, the main fluorescent compound, was 
determined to be C10H8O4 having a molecular weight of 192.17. 
Significant differences were noted between the remedies prepared in 
the two types of containers. Further, a comparison between any two 
methods of agitation revealed significant differences in fluorometric 
data of remedies at certain potency levels. The biological 
(anticancer) action of the crude extract, the alkaloid scopoletin, 
and 2C potency of Gelsemium sp were tested in vitro on the HeLa cell 
line through fluorescence microscopy, the 3(4, 
5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, 
and fluorescent activated cell sorting (FACS). The role of 
nanoparticles presumably derived from the containers, their 
orientation, and their interaction with the starting substance during 
the dynamization process initiated by different modes of agitation 
could possibly be attributed to the differences noted in the 
fluorometric data of potencies prepared in the two types of 
containers and among the three different means of succussion tested.

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