<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""> <div class=""><b class=""> Comparison between the additive effects of diluted (rFSH) and diluted/dynamized (FSH 6 cH) recombinant follicle-stimulating hormone on the in vitro culture of ovine preantral follicles enclosed in ovarian tissue<br class=""></b>L.F. Lima, R.M.P. Rocha, A.M.C.V. Alves, A.A. Carvalho, R.N. Chaves, C.A.P. Lopes, S.N. Báo, C.C. Campello, A.P.R. Rodrigues, J.R. Figueiredo<br class=""><br class="">Objective - This study compared 2 types of recombinant follicle stimulating hormone (rFSH): diluted and diluted/dynamized, on in vitro development of ovine follicles.<br class="">Methods - In experiment 1, ovarian fragments were cultured for 1 or 7 days in α-MEM+ in the absence or presence of different concentrations of diluted rFSH to determine the best concentration. In experiment 2, the effect of diluted and diluted/dynamized rFSH (rFSH 6 cH—ultradiluted and succussioned), alone or in combination, was studied.<br class="">Results - In experiment 1, compared to control, 50 ng/mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture (P < 0.05). In experiment 2, compared to control, diluted/dynamized rFSH induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture (P < 0.05). Compared to diluted rFSH, diluted/dynamized rFSH induced higher rates of follicle activation at day 1 of culture (P < 0.05).<br class="">Conclusion - In conclusion, compared to the control medium, diluted/dynamized rFSH promoted survival and early activation of follicles, while diluted rFSH promoted higher activation later in the culture. Thus, diluted/dynamized rFSH may be used as an alternative to diluted rFSH for the in vitro culture of ovine preantral follicles.<br class=""><br class="">Disponibile il full-text su richiesta<br class=""> </div> </body></html>