[Omeo33] Art 1692 - Homeopathy, 2017, 106 (4), 203-213

[Tiziana Di Giampietro]

Vorrei questo full text
Grazie
Tiziana

Il giorno 28 febbraio 2018 10:12, Gino Santini <segreteria.ismo a gmail.com>
ha scritto:

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> SIOMI / Redazione di Omeopatia33
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> * Therapeutic potential of HIV nosode 30c as evaluated in A549 lung cancer
> cells*Anisur Rahman Khuda-Bukhsh, Jesmin Mondal, Rajesh Shah
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> Objectives - To examine if HIV nosode in 30c dilution (HIV 30c) has
> therapeutic potential against lung cancer cells (A549) as compared to
> WRL-68 normal cells and to elucidate its possible molecular mechanism of
> action on DNA replication and apoptosis.
> Methods - Effects of HIV 30c were thoroughly tested for its possible
> anticancer potential on A549 cells (lung cancer); WRL-68 normal liver cells
> served as control. Three doses, one at LD50 and two below LD-50, were used.
> Proliferation, migration and senescence assays were made and generation of
> reactive oxygen species (ROS) studied by routine techniques. The ability of
> HIV 30c to induce apoptosis in A549 cells and its possible signalling
> pathway were determined using immunoblots of relevant signal proteins and
> confocal microscopy, including studies on telomerase reverse transcriptase
> (TERT) and topoisomerase II (Top II) activities, intimately associated with
> cell division and DNA replication.
> Results - HIV 30c prevented cancer cell proliferation and migration,
> induced pre-mature senescence, enhanced pro-apoptotic signal proteins like
> p53, bax, cytochrome c, caspase-3 and inhibited anti-apoptotic signal
> proteins Bcl2, TERT and Top II, changed mitochondrial membrane potential
> and caused externalization of phosphatidyl serine. Thus, it induced
> apoptosis as also evidenced from increase in cells with distorted membrane
> morphology, nuclear condensation, DNA fragmentation, and ROS, typical of
> apoptosis in progress.
> Conclusion - HIV 30c nosode has therapeutic potential for inducing
> cytotoxic effects on A549 cells as manifested by changes in nuclear
> condensation, DNA fragmentation, ROS generation and MMP, and for its
> inhibitory action on cell proliferation, cell migration, expression of
> telomerase reverse transcriptase and Top II genes, and increasing
> expression of pro-apoptotic genes.
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