[Omeo33] Art 1546 - Compl Ther Med, 2016, 25, 39-44

[Gino Santini]

Comparison between the additive effects of diluted (rFSH) and diluted/dynamized (FSH 6 cH) recombinant follicle-stimulating hormone on the in vitro culture of ovine preantral follicles enclosed in ovarian tissue
L.F. Lima, R.M.P. Rocha, A.M.C.V. Alves, A.A. Carvalho, R.N. Chaves, C.A.P. Lopes, S.N. Báo, C.C. Campello, A.P.R. Rodrigues, J.R. Figueiredo

Objective - This study compared 2 types of recombinant follicle stimulating hormone (rFSH): diluted and diluted/dynamized, on in vitro development of ovine follicles.
Methods - In experiment 1, ovarian fragments were cultured for 1 or 7 days in α-MEM+ in the absence or presence of different concentrations of diluted rFSH to determine the best concentration. In experiment 2, the effect of diluted and diluted/dynamized rFSH (rFSH 6 cH—ultradiluted and succussioned), alone or in combination, was studied.
Results - In experiment 1, compared to control, 50 ng/mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture (P < 0.05). In experiment 2, compared to control, diluted/dynamized rFSH induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture (P < 0.05). Compared to diluted rFSH, diluted/dynamized rFSH induced higher rates of follicle activation at day 1 of culture (P < 0.05).
Conclusion - In conclusion, compared to the control medium, diluted/dynamized rFSH promoted survival and early activation of follicles, while diluted rFSH promoted higher activation later in the culture. Thus, diluted/dynamized rFSH may be used as an alternative to diluted rFSH for the in vitro culture of ovine preantral follicles.

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