[Omeo33] Art 1319 - Homeopathy, 2013, 102 (1), 41-48

[Gino Santini]

Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro
Laritza Ferreira de Lima, Rebeca Magalhães Pedrosa Rocha, Anelise Maria Costa Vasconcelos Alves, Márcia Viviane Alves Saraiva, Valdevane Rocha Araújo, Isadora Machado Teixeira Lima, Cláudio Afonso Pinho Lopes, Sônia Nair Báo, Claudio Cabral Campello, Ana Paula Ribeiro Rodrigues, José Ricardo de Figueiredo

Objective

To evaluate the effect of dynamized follicle-stimulating hormone (FSH) on the survival, activation and growth of ovine preantral follicles (PFs) in vitro.
Methods

Ovarian fragments were cultured for 1 or 7 days in alpha minimum essential medium (α-MEM+) control in the absence or presence of alcohol (Al control) or FSH (6cH, 12cH and 30cH) added at intervals of 24 or 48 h. The ovarian fragments were processed, coded and analyzed by a blinded observer by classical histology (CH), fluorescence microscopy (FM) and transmission electron microscopy (TEM).
Results

After 7 days of culture, the group which to which FSH 6cH was added at 24 h intervals showed better rates of follicle survival and activation compared to α-MEM+ control or Al control (p < 0.05). This group also showed higher follicle and oocyte growth than α-MEM+ control (p < 0.05). FM and TEM techniques confirmed that FSH 6cH promoted viability and ultrastructural integrity of follicles after 7 days of culture.
Conclusions

FSH 6cH (24 h) treatment maintained the viability, and promoted the activation and in vitro growth of ovine PFs.

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