[Omeo33] Art 0050 - Homeopathy, 2005, 94 (4), 229-232

[Gino Santini]

Stimulation of bovine sperm mitochondrial activity by homeopathic  
dilutions of monensin
DM Aziz and H Enbergs

Mitochondrial activity is an important viability parameter of  
spermatozoa and is linked to sperm motility. Monensin is commonly used  
as an inhibitor for sperm mitochondrial activity in the laboratory.  
This study was conducted to evaluate the influence of some homeopathic  
dilutions of monensin on sperm mitochondrial activity. Fresh  
ejaculates from 6 mature bulls were used in the study. Samples of the  
semen were tested using a flow cytometer for mitochondrial activity  
and sperm viability using Rhodamine 123 and SYBR-14, respectively. The  
9X dilution of monensin resulted in very highly significant (P<0.001)  
stimulation of mitochondrial activity. Monensin 5X, 7X, 8X and 13X  
caused highly significant (P<0.01) stimulation of the sperm  
mitochondrial activity. Other homeopathic dilutions of monensin (6X,  
10X, 11X, 12X and 14X) also had a significant (P<0.05) stimulatory  
effect. The use of monensin did not have any negative effect on sperm  
viability. We conclude that some homeopathic dilutions of monensin  
increase mitochondrial activity of bovine spermatozoa without negative  
effect on sperm viability, the 9Å~ dilution was the most effective.  
Further in vivo studies are required to estimate the effect of  
homeopathic dilutions of monensin on semen quality.

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