[Omeo33] Art 0955 - Exp Biol Med, 2008, 233 (12), 1591-1601

[Gino Santini]

In Vitro Studies Demonstrate Anticancer Activity of an Alkaloid of the  
Plant Gelsemium sempervirens
Soumya Sundar Bhattacharyya, Sushil Kumar Mandal, Raktim Biswas, Saili  
Paul, Surajit Pathak, Naoual Boujedaini, Philippe Belon and Anisur  
Rahman Khuda-Bukhsh

The chemical structure of the main fluorescenting compound in the  
ethanolic extract (mother tincture) of the American yellow jasmine,  
Gelsemium sempervirens, was determined by employing 1H nuclear  
magnetic resonance (NMR), 13C NMR, mass spectroscopy, high-performance  
liquid chromatography (HPLC), correlation spectroscopy (COSY), and  
Fourier transform infrared (FTIR) spectroscopy analyses.  
Spectrofluorometric analysis has been made of the mother tincture and  
its agitated serial dilutions (up to 12th potency) prepared according  
to a homeopathic procedure in which serial, agitated dilutions were  
made separately in glass and polypropylene containers. The succussions  
were made by employing three different modes: hand jerk, sonication,  
and vortexing. The chemical formula of scopoletin, the main  
fluorescent compound, was determined to be C10H8O4 having a molecular  
weight of 192.17. Significant differences were noted between the  
remedies prepared in the two types of containers. Further, a  
comparison between any two methods of agitation revealed significant  
differences in fluorometric data of remedies at certain potency  
levels. The biological (anticancer) action of the crude extract, the  
alkaloid scopoletin, and 2C potency of Gelsemium sp were tested in  
vitro on the HeLa cell line through fluorescence microscopy, the 3(4,  
5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay,  
and fluorescent activated cell sorting (FACS). The role of  
nanoparticles presumably derived from the containers, their  
orientation, and their interaction with the starting substance during  
the dynamization process initiated by different modes of agitation  
could possibly be attributed to the differences noted in the  
fluorometric data of potencies prepared in the two types of containers  
and among the three different means of succussion tested.

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